Voltammetric determination of copper and zinc in water using a Ruthenium bipyridyl/Nafion-modified indium tin oxide-coated glass electrode

  • Shirley Tiong Palisoc Condensed Matter Research Unit, Center for Natural Science and Environmental Research, College of Science, De La Salle University, 2401 Taft Avenue, Manila 0922, Philippines
  • Michelle Tiamzon Natividad De La Salle University
  • Craig Egan Allistair Dumanon Tan Condensed Matter Laboratory, Physics Department, De La Salle University, 2401 Taft Ave., Manila, Philippines
Keywords: Nafion, Ruthenium bipyridyl, Indium Tin Oxide, Anodic Stripping Voltammetry, Copper, Zinc


Chemically-modified Indium Tin Oxide (ITO) coated glass substrates were fabricated by depositing Nafion thin films doped with tris (2,2’-bipyridyl) ruthenium(II) dichloride hexahydrate via drop coating deposition technique. The Nafion volume concentration in the film is 1% (v/v) and the mediator concentration is 5 mg per 1 mL Nafion. The chemically-modified electrodes were characterized by cyclic voltammetry (CV) and were used as working electrodes to detect varying concentrations of copper (Cu2+) and zinc (Zn2+) in a 0.1 M NaCl supporting electrolyte solution via Anodic Stripping Voltammetry (ASV). Cu2+ and Zn2+ ions were successfully determined by ASV. The limits of detection for Cu2+ and Zn2+ were 0.1 ppm and 0.7 ppm, respectively. The modified electrodes were used to determine the presence of Cu2+ and Zn2+ in different real water samples. The presence of Cu2+ was successfully determined in deep well, lake, and tap water samples, while the presence of Zn2+ was successfully determined in sea and tap water samples. Atomic Absorption Spectroscopy (AAS) results confirm the presence of Cu2+ and Zn2+ in the samples.


[1] M. Wilhelm, B. Heinzow, J. Angerer, C. Schulz, Int. Jour. of Hyg. and Env. Hea., 213, 265 (2010).
[2] H.J. Sun, B. Rathinasabapathi, B. Wu, J. Luo, L.P. Pu, Env. Int., 69, 148 (2014).
[3] L.M. Gaetke, C.K. Chow, Tox., 189, 147 (2003).
[4] World Health Organization, Copper in Drinking Water, Retrieved January 25, 2015 from http://www.who.int/water_
[5] K. Dzieżyc, T. Litwin, G. Chabik, A. Członkowska, Gait & Posture, 42, 601 (2015).
[6] G. Kovacs, N. Montalbetti, M.C. Franz, S. Graeter, A. Simonin, M.A. Hediger, Cell Cal., 54, 276 (2013).
[7] World Health Organization. Zinc in Drinking Water. Retrieved April 7, 2015 from http://www.who.int/water_sanitation_health
[8] J. Lee, Y.S. Yim, S.J. Ko, D.G. Kim, C.H. Kim, Bra. Res. Bull., 86, 314 (2011).
[9] B.C. Janegitz, L.H. Marcolino-Junior, S.P. Campana-Filho, R.C. Faria, O. Fatibello-Filho, Sens. and Act. B, 142, 260 (2009).
[10]L. Zhu, L. Xu, B. Huang, N. Jia, L. Tan, S. Yao, Elec. Act., 115, 471 (2014).
[11]S. Legeai, O. Vittori, Anal. Chim. Act., 560, 184 (2006).
[12]F. Torma, M. Kádár, K. Tóth, E. Tatár, Anal. Chim. Act., 619, 173 (2008).
[13]Y. Song, G.M. Swain, Anal. Chim. Act., 593, 7 (2007).
[14]B. Kong, B. Tang, X. Liu, X. Zeng, H. Duan, S. Luo, W. Wei, Jour. of Haz. Mat., 167, 455 (2009).
[15]P. Sonthalia, E. McGaw, Y. Show, G.M. Swain, Talanta, 64, 562 (2004).
[16]A. Giacomino, O. Abollino, M. Lazzara, M. Malandrino, E. Mentasti, Talanta, 83, 1428 (2011).
[17]Q. Duan, H. Wang, J. Benziger, Jour. of Mem. Sci., 392, 88 (2012).
[18]P. Bertencelllo, N.R. Wilson, P.R. Unwin, Soft Matter, 3, 1300 (2007).
[19]S.T. Palisoc, M.T. Natividad, N.M.D.C. Martinez, R.M.A. Ramos, K.A.Y. Kaw, e-Polymers, 16, 117 (2015).
[20]S.T. Palisoc, M.T. Natividad, P.D. DeVera, B.S.B. Tuason, Phil. Sci. Lett., 7, 372 (2014).
[21]S.T. Palisoc, M.T. Natividad, P.D. DeVera, B.S.B. Tuason, J. N. Mat. Electr. Sys., 17, 205 (2014).
[22]S.T. Palisoc, B.S.B. Tuason, M.T. Natividad, Opto. and Adv. Mat. – Rap. Comm., 9, 1435 (2015).
[23]S.T. Palisoc, M.T. Natividad, F. Co, K.A.Y. Kaw, Optoelectron. Adv. Mat., 9, 1010 (2015).
[24]S. Kádár, T. Amemiya, K. Showalter, J. Phys. Chem. A., 101, 8200 (1997).
[25]K.A.Y.Kaw, S.T. Palisoc, M.T. Natividad, Phil. Sci. Lett., 7, 171 (2014).
[26]C. Zhao, L. Xing, J. Xiang, L. Cui, J. Jiao, H. Sai, Z. Li, F. Li, Part., 17, 66 (2014).
[27]Y. Liu, Y. Han, X. Zhao, Y. Tong, Q. Tang, Y. Liu, Syn. Met., 198, 248 (2014).
[28]Skoog, D.A., Holler, F.J., and Nieman, T.A., Principles of instrumental analysis, 5, 13 (1998).
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